CONTACT US

Our Address

Science Avenue, High-tech Zone, Zhengzhou City, Henan Province, China

  • In-Cell ELISA protocol Abcam

    Discover our In-Cell ELISA protocol, used to measure protein levels or post-translational modifications of cultured adherent cells. Fix cells to microplate Immediately add an equal volume (100μL) of 8% paraformaldehyde solution to the wells containing culture media.

  • Immunofluorescence Protocol (IF Protocol) Sino

    Immunofluorescence is one of the widely used techniques in modern biology and medicine, and it is developed by Coons et al. (1950), and it is a combination of immunofluorescence technique and morphological technology to develop immune fluorescent cells (or tissue).

  • Basic Guidelines and Protocols School of Medicine ...

    2020-7-19  Protocols. Protein Precipitation . FASP (Filter-Aided Sample Prep) In-solution Tryptic Digestion . In-gel Tryptic Digestion . SDS-PAGE protein separation . SILAC media preparation . Basic Guidelines for Protein Sample Handling and Preparation. High purity preparation of a protein sample (or protein complex) is the key to successful mass ...

  • Protocols:Protein Micro-Contact Printing - DJGroup

    2015-1-12  Place PDMS squares in beaker filled with 70% Ethanol in the chemical hood for 24 hours Dry PDMS squares with Nitrogen gun Place in the oven overnight at 60ºC Printing of Protein. Cut PDMS squares again into quarters (0.5cm x 0.5cm) Wash with ethanol for 5 min in the ultrasonic bath at 35 kHz and maximum power Dry thoroughly with nitrogen gas

  • Flow hood -Bio-thing

    2018-9-5  [Abstract] Proper spatiotemporal regulation of protein phosphorylation in cells and tissues is required for normal development and homeostasis. We present the protocol ‘In Gel Kinase Assay’, which is useful for protein kinase activity measurements from crude protein extracts.

  • Current Protocols in Protein Science Unit 26.5 (ms# CP

    位置: 8600 Rockville Pike, Bethesda, MD
  • Electroelution of Proteins from Polyacrylamide Gels ...

    Abstract. One- or two-dimensional polyacrylamide gel electrophoresis (PAGE) is one of the most versatile methods for protein separation. The resolving power of a two-dimensional O’Farrell gel (1 and see Chapter 20–22) cannot be reached with today’s high-performance liquid chromatographic techniques unless specific affinity interactions are being exploited for protein purification.

  • Carl Zeiss Microscopy LCM Protocols – Protein Handling

    2014-9-4  LCM Protocols - Protein Handling for LC/MS LCM Protocols - Protein Handling for LC/MS The DOs and DON’Ts of handling proteins Contamination is a common reason for failing experiments. Keratins from the skin are prone to contaminate the sample and obscure the proteins of interest.

  • Protocol for Culturing Rat Hippocampal Neurons: RD

    Note: Preparation of the culture plates should be done in a laminar flow cell culture hood. Dilute the Cultrex ® Poly-D-Lysine solution with sterile dH 2 O to a final concentration of 50 µg/mL. Cover the wells of the culture plates with 50 µg/mL Cultrex ® Poly-D-Lysine solution (e.g., 50 µL/well for 96-well plate).

  • Flp-In System: For Generating Constitutive Expression

    Protein Expression Protocols ... Weigh out blasticidin and prepare solutions in a hood. Preparing and Storing Stock Solutions Blasticidin may be obtained separately from Invitrogen (Catalog no. R210-01) in 50 mg aliquots. Blasticidin is soluble in water. Sterile water is

  • Electroelution of Proteins from Polyacrylamide Gels ...

    Abstract. One- or two-dimensional polyacrylamide gel electrophoresis (PAGE) is one of the most versatile methods for protein separation. The resolving power of a two-dimensional O’Farrell gel (1 and see Chapter 20–22) cannot be reached with today’s high-performance liquid chromatographic techniques unless specific affinity interactions are being exploited for protein purification.

  • Current Protocols in Protein Science: Vol 7, No 1

    1997-7-1  Click on the title to browse this issue

  • Flp-In System: For Generating Constitutive Expression

    Protein Expression Protocols ... Weigh out blasticidin and prepare solutions in a hood. Preparing and Storing Stock Solutions Blasticidin may be obtained separately from Invitrogen (Catalog no. R210-01) in 50 mg aliquots. Blasticidin is soluble in water. Sterile water is

  • Western Blotting Protocols - Western Blotting - 资讯 - 生物在线

    2010-8-22  Preparation of Protein Blots for Long-term Storage PVDF is a chemically resistant polymer with excellent long term stability. For blots that need to be stored for use at a later date, storage conditions are determined by the stability of the proteins bound to the membrane.

  • Flow hood -Bio-thing

    2018-9-5  [Abstract] Proper spatiotemporal regulation of protein phosphorylation in cells and tissues is required for normal development and homeostasis. We present the protocol ‘In Gel Kinase Assay’, which is useful for protein kinase activity measurements ...

  • Protocols for Membrane Scaffold Proteins and

    2020-7-25  One fairly common question relates to protocols that incorporate more than one protein into a Nanodisc. Several references discuss this question with respect to the assembly of bacteriorhodopsin for some phospholipids. 3-6 The procedure is facilitated by the use of

  • Sample extraction techniques for ... - Nature Protocols

    Many protein extraction protocols have been developed 4, and choosing an appropriate protocol largely depends on the nature of the extracted tissue and on the downstream application (e.g ...

  • Protein Expression in the Baculovirus System_百度文库

    Protein Expression in the Baculovirus System 5.5.10 Current Protocols in Protein Science To harvest by tangential flow microfiltration (>20 liters) 1c. Connect the inlet of

  • Protein Extraction and Solubilization using the TRIZOL ...

    2016-7-9  Extracting protein from tissue samples and cultured cells is Step #1 in many biochemical and analytical techniques. Before you can do a Polyacrylamide Gel Electrophoresis (PAGE), a Western blot, or mass spectrometry you need to extract your protein. Nowadays, a lot of labs have switched to kits for their protein extraction but these kits can be more expensive and can have poorer protein yields ...

  • Analysis of protein glycosylation by ... - Nature Protocols

    2007-6-21  We present a detailed protocol for the structural analysis of protein-linked glycans. In this approach, appropriate for glycomics studies, N-linked glycans are released using peptide N

  • Quantifying Plant Soluble Protein ... - Methods and

    The protocols described herein provide a clear and approachable methodology for measuring soluble protein and digestible (non-structural) carbohydrate content in plant tissues. The ability to quantify these two plant macronutrients has significant implications for advancing the fields of plant physiology, nutritional ecology, plant-herbivore ...

  • Western Blotting Protocols Life Science Research

    Incubating the membrane with primary antibody, which binds to the protein of interest; ... Two protocols for removing antibodies from blots are presented below. The first is applicable to any chemiluminescent substrate system and uses a combination of detergent and heat to release the antibodies. ... In a fume hood, place the blot in stripping ...

  • Using Biacore to Measure the Binding ... - Current

    The optical phenomenon of surface plasmon resonance (SPR) used by Biacore systems enables the detection and measurement of protein‐protein interactions in real time, without the use of labels. In this unit, the application of Biacore technology to measure a protein‐protein interaction is described using an antibody and its antigen as an ...

  • Analysis of protein glycosylation by ... - Nature Protocols

    2007-6-21  We present a detailed protocol for the structural analysis of protein-linked glycans. In this approach, appropriate for glycomics studies, N-linked glycans are released using peptide N

  • ICCVAM-Recommended Test Method Protocol The

    2020-7-20  ICCVAM-Recommended Test Method Protocol The Human Whole Blood/Interleukin-1β In Vitro Pyrogen Test Application of Cryopreserved Human Whole Blood Originally published as Appendix C2 of “ICCVAM Test Method Evaluation Report:

  • A novel protein refolding protocol for the

    2012-4-1  Highlights We developed a promising protein refolding protocol for an outer membrane protein. The protocol was effective for the solubilization and purification of XfPal. High yield of purified protein with secondary and tertiary structure was obtained. The refolded XfPal was able to interact in vitro with peptidoglycan. This protocol is valuable for future structural and functional studies of ...

  • Protein Extraction and Solubilization using the TRIZOL ...

    2016-7-9  Extracting protein from tissue samples and cultured cells is Step #1 in many biochemical and analytical techniques. Before you can do a Polyacrylamide Gel Electrophoresis (PAGE), a Western blot, or mass spectrometry you need to extract your protein. Nowadays, a lot of labs have switched to kits for their protein extraction but these kits can be more expensive and can have poorer protein yields ...

  • DNA Extraction Protocols Thermo Fisher Scientific - US

    A collection of DNA Extraction Protocols for research, provided by Invitrogen

  • 2015 His tag Purification Protocol - University of San Diego

    2015-6-3  PROVOST’’WALLERT’RESEARCH! Investigating!the!Biochemistry!! Cellular!Physiology!of!NHE1! EST.%1998! His Tag Purification Purification Protocol

  • Yeast Protocols Handbook_百度文库

    2010-7-7  Yeast Protocols Handbook - User Manual Yeast Protocols Handbook FOR RESEARCH USE ONLY United St... 百度首页 登录 加入VIP 享VIP专享文档下载特权 赠共享文档下载特权 100w优质文档免费下载 赠百度阅读VIP精品版 立即开通 意见反馈 下载客户端 ...

  • (PDF) Current Protocols in Protein Science

    Current Protocols in Protein Science Supplement 69 Overlay A +L5 +N3 Figure 14.13.4 Histochemical staining of nitrotyrosine in fresh frozen liver tissue sections from mice treated with CCl 4 .

  • LCM Protocols and Techniques

    2013-8-22  − Staining agents and protocols can affect quality and yield of recovered molecules − Some samples do not require staining –ex. GFP label •Dehydration Removal of water inactivates nucleases > Graded ethanol series –75%, 95%, 100% > Xylene steps

  • Target identification using drug affinity responsive ...

    位置: 8600 Rockville Pike, Bethesda, MD
  • Yeast Two-Hybrid System for Studying Protein 冷泉港

    2019-7-14  Protocols for stages 2-4 of the method are described inYeast Two-Hybrid System for Studying Protein-Protein Interactions--Stage 2: ... Fume hood Gloves Heat block preset to 100°C or boiling water bath Another alternative is to use a polymerase chain reaction ...

  • Electrophoresis Protocols National Diagnostics

    To prepare 200 ml of ProtoBlock solution, add 10 grams of ProtoBlock Reagent A to 170 ml of deionized water. Mix well and add 20 ml of ProtoBlock Reagent B. This solution is used to block the membrane after protein transfer and to dilute the antibodies used in the immunoassays. If any blocking solution remains, store at 4 o C for future use.

  • A novel protein refolding protocol for the

    2012-4-1  Highlights We developed a promising protein refolding protocol for an outer membrane protein. The protocol was effective for the solubilization and purification of XfPal. High yield of purified protein with secondary and tertiary structure was obtained. The refolded XfPal was able to interact in vitro with peptidoglycan. This protocol is valuable for future structural and functional studies of ...

  • Scientific Protocols - Expression and accumulation of ...

    Protocol; Discussion; Authors: Giovanni Vianna, Nicolau da Cunha Elíbio Rech Abstract. The production of recombinant molecules in seeds plant has presented interesting results related to the synthesis, secretion, compartmentalisation, post-translational modifications and purification, scalability, stable expression, the absence of contaminants and human pathogens, and knowledge of the ...

  • DNA Extraction Protocols Thermo Fisher Scientific - US

    A collection of DNA Extraction Protocols for research, provided by Invitrogen

  • Protocol - ELISPOT Protocol - BioLegend

    Phosphate Buffered Saline (PBS): 80.0g NaCl 14.4g Na 2 HPO 4, 2.4g KH 2 PO 4, 2.0g KCl Add ddH 2 O up to 10L; pH to 7.2 with HCl; Coating Buffer: Can use either Sterile PBS or Sterile Carbonate Buffer (per ELISA protocol) 8.4g Na HCO 3, 3.56g Na 2 CO 3, add ddH 2 O up to 1.0L, pH to 9.5.; PBS-Tween: 0.05% Tween-20 in PBA (500µl Tween-20 in 1L PBS)

  • MTT Proliferation Assay Protocol - University of San Diego

    2015-6-16  PROVOSTWALLERTRESEARCH! Investigating!the!Biochemistry!! Cellular!Physiology!of!NHE1! EST.%1998! MTT Proliferation Assay Protocol ! 2 June 15 Note: For most tumor cells, hybridomas, and fibroblast cell lines, 5,000 cells per well to perform proliferation assays.12 well plates need 1 ml of soln and have about 400,000 cells, 24 well plates have 0.5 ml and 200,000